#tleap.in
source leaprc.protein.ff19SB
source leaprc.water.opc
loadamberparams frcmod.ions1lm_126_hfe_opc
ramp=loadpdb RAMP1.pdb
bond ramp.27.SG ramp.82.SG
bond ramp.40.SG ramp.72.SG
bond ramp.57.SG ramp.104.SG
SaveAmberParm ramp RAMP1_gas.prmtop RAMP1_gas.inpcrd
addIons ramp Na+ 2
solvateOct ramp OPCBOX 10.0
addIonsRand ramp Na+ 19 Cl- 19 
SaveAmberParm ramp RAMP1_ion.prmtop RAMP1_ion.inpcrd
